Joint Expeditionary Base–Little Creek (JEB–LC), formerly known as Naval Amphibious Base Little Creek and commonly called simply Little Creek, is the major operating base for the Amphibious Forces in the United States Navy's Atlantic Fleet. The base comprises four locations in three states, including almost 12,000 acres (4,900 ha) of real estate. Its Little Creek location in Virginia Beach, Virginia totals 2,120 acres (860 ha) acres of land. Outlying facilities include 350 acres (140 ha) located just north of Training Support Center Hampton Roads in Virginia Beach, and 21 acres (8.5 ha) known as Radio Island at Morehead City, North Carolina, used as an amphibious embarkation/debarkation area for U.S. Marine Corps units at Marine Corps Base Camp Lejeune, North Carolina.

The mission of the Naval Amphibious Base is to provide required support services to over 15,000 personnel of the 27 homeported ships and 78 resident and/or supported activities. The base's combination of operational, support, and training facilities are geared predominantly to amphibious operations, making the base unique among bases of the United States and Allied Navies. The Naval Amphibious Base Little Creek is the largest base of its kind in the world.

On October 1, 2009, Little Creek and the Army's Fort Story finished a two-year merge into one joint base, officially named Joint Expeditionary Base Little Creek–Fort Story.